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Fig. 3. Dystroglycan and Perlecan can bind in vitro. Western blots showing co-immunoprecipitation of PcanV with anti-Dg^pep. Embryonic glycoprotein extract was mixed with 5 µg PcanV (R1), 0.5 µg PcanV (R2) or 0.05 µg PcanV (R3), and precipitated with anti-Dg^pep. Controls were: no glycoprotein extract added to reaction mixture (C1), precipitation reaction with boiled Dg antibody (C2), only primary antibody loaded (C3), primary antibody exchanged for rabbit serum (C4). The precipitates were probed with anti-PcanV (upper panel) or anti-Dg^cyto (lower panel). Pure PcanV (PcanV 0.05 µg) and glycoprotein extract (input) were loaded as a positive control. Some of the minor Dg bands in the glycoprotein extract (input) are probably due to protein degradation.

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