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Figure 3


Fig. 3. PKD-2::GFP localization is altered in unc-101, lov-1 and IFT mutants. (A,D,G,J) Cartoons of a sensory neuron expressing PKD-2::GFP (shown in green) for each genotype. (B,E,H,K) CEMs, (C,F,I,L) RnBs and HOB neurons. (A-C) Wild-type localization of PKD-2::GFP. PKD-2 in ciliary endings (ciliary membrane and ciliary base, arrowheads) in CEMs and RnBs. (D-F) In unc-101(m1) mutants, PKD-2::GFP is uniformly distributed throughout neurons, including axons, cell bodies, dendrites (thick arrows) and cilia (arrowhead). (G-I) In lov-1(sy582) mutants, PKD-2::GFP aggregates within the cell bodies (arrows) and is expressed in a lower level in ray cilia (arrowhead in 3I). The boxed inset in I shows a closer view of a ray cell body containing PKD-2::GFP aggregates. (J-L) In daf-10(p821) mutants, PKD-2::GFP accumulates in the ciliary base (arrowheads). In the RnBs, PKD-2::GFP also mislocalizes to dendrites (thick arrow). This mutant phenotype is consistent in other IFT single and double mutant backgrounds. Scale bars: 10 µm.





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