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Fig. 4. PKD-2::GFP abundance in cilia is altered in lov-1 and IFT mutants. (A,B) Fluorescence intensity in CEM cilia (F_cilia) shows that PKD-2::GFP accumulates in IFT mutants. (A) F_cilia of PKD-2::GFP in CEMs in wild-type, lov-1, daf-10 and osm-5 backgrounds. F_cilia in the IFT mutants (daf-10 and osm-5) is approximately three times greater than in wild type. (B) Ratio (F_cilia/F_{cell body}) shows that PKD-2::GFP abundance in CEM cilia is increased in comparison with the cell bodies in IFT mutants (daf-10 and osm-5). (C-E) Fluorescence intensity measurement in RnB ray cilia of wild type and lov-1 mutants reveals reduced ciliary localization in lov-1 background. (C) PKD-2::GFP localization in RnB ray cilia is often below detectable levels in lov-1 mutants. R1B to R5B are selected because they are easily distinguished from other rays. The percentage reflects the number of rays exhibiting detectable ciliary localization divided by total number of ray pairs scored. Those cilia with detectable GFP were used for ciliary measurement in D and E. (D) F_cilia in RnB cilia showed that PKD-2::GFP levels in lov-1 mutants are significantly reduced. (E) F_cilia/F_{cell body} ratio in rays reveals decreased PKD-2::GFP ciliary abundance in lov-1 mutants. Error bars indicate s.e.m. Non-parametric Mann-Whitney tests with two-tailed P-value were performed between wild type and each genotype. ns, not significant (P>0.05); ^***P<0.001; n, number of cilia measured.

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