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Figure 1


Fig. 1. A human DBH promoter-directed transgene recapitulates endogenous Gata3 expression in the developing sympathoadrenal system. (A) Diagrammatic representations of the mouse Gata3 gene, the Gata3 knockout (Pandolfi et al., 1995), the Gata3 nuclear lacZ knock-in (van Doorninck et al., 1999) and the hDBH-Gata3 expression transgene (TghDBH-G3) and co-injected hDBH-GFP transgene. X, XhoI restriction enzyme site. (B) Genomic Southern blot showing the diagnostic XhoI genomic DNA fragments corresponding to wild-type (WT), Gata3/lacZ knock-in (KI), Gata3 knock-out (KO) and hDBH-Gata3 transgene (TghDBH-G3) alleles. (C-K) Anti-GFP and anti-ß-galactosidase immunoreactivities in the adrenal medulla (AM), thoracic paravertebral sympathetic ganglia (SG) and organ of Zuckerkandl (OZ) in E18.5 Gata3Z/+:TghDBH-G3 embryos. Scale bars: 100 µm.





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