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Fig. 4. Phox2b and Th expression in thoracic paravertebral sympathetic ganglia
is suppressed in E18.5 Gata3 mutant embryos. (A-C)
Whole-mount X-gal staining of E18.5 Gata3Z/+ and
Gata3Z/- embryos with and without TghDBH-G3
transgene. ß-Gal+ sympathetic chain was shrunken in the
pharmacologically rescued Gata3Z/- embryos.
(D,F) The size of sympathetic ganglion is reduced in the
pharmacologically rescued Gata3-/- embryos in HE sections.
In Gata3Z/-:TghDBH-G3 embryos, anti-Phox2b and
anti-Th staining and sympathetic ganglion size were restored
(B,E,H,K); the control animals used were
Gata3Z/+ (A) and Gata3+/+ (WT;
wild-type) littermates (D,G,J). Broken lines indicate outer
margins of sympathetic ganglia. (I,L) In
Gata3-/- embryos, Phox2b and Th expression was
significantly reduced in thoracic paravertebral sympathetic ganglia. Scale
bar: 100 µm. (M,N) At least four serial sections of each of
the four examined ganglia from two different embryos of each genotype were
evaluated. Phox2b- and Th-expressing cells (M) and the total surface areas (N)
of the thoracic paravertebral sympathetic ganglia in control and
Gata3 mutant embryos with and without TghDBH-G3 were
quantified and represented as histogram. Data are presented as the
mean±s.e.m. The statistical significance of the differences between
Gata3-/-:TghDBH-G3 and
Gata3-/- are indicated (*P<0.05;
**P<0.01; Student's t-test).
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