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Fig. 6. Activity of the non-MES-4 H3K36 HMT(s) depends on transcription.
Analysis of H3K36me2 levels in
100-cell embryos lacking MES-4, AMA-1, or
both. DNA is stained red with PA3; H3K36me2, green. (A) Wild-type
embryo. (B,C) mes-4(bn73) and ama-1(RNAi)
embryos display reduced H3K36me2 relative to wild type. (D) The
mes-4(bn73); ama-1(RNAi) embryo lacks detectable H3K36me2
signal. Scale bar: 10 µm.