DEV02538 Supplementary Material

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• Supplemental Table -

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• Supplemental Figure 1 -

  Fig. S1. lacZ expression in various adult tissues. Whole-mount X-gal staining for lacZ activity in Man1 heterozygous 11-week-old mice. (A) Testis, (B) thyroid and parathyroid glands, (C) adrenal glands, (D,E) spleen, (F,G) cerebrum, (H) cerebellum, (I) trachea, and (J) heart. E and G are higher magnification views of the boxed areas in D and F, respectively. All sections were counterstained with Eosin after X-gal staining. Sg, spermatogonia; Ty, thyroid gland; pTy, parathyroid gland; Cor, cortex; Med, medulla; EC, endothelial cells; VSMC, vascular smooth muscle cells; SS, somatosensory cortex; Py, layer V pyramidal cell; GL, granular layer; Pu, Purkinje cells; Ch, chondrocytes; SM, smooth muscle cells; My, myocardium. Scale bars: 100 μm in A-D,F, H; 50 μm in E,G,I,J.

• Supplemental Figure 2 -

  Fig. S2. Augmentation of the Smad2/3 pathway in neural and endothelial cells of Man1^Δ/Δ embryos. Confocal microscopic analyses of the neural (A-J,A′-J′) and endothelial cells (K-T,K′-T′) of wild-type and Man1^Δ/Δ embryos at E9.5 for PS1 and PS2. Nuclei were visualized with DAPI. Higher magnification images of the insets in A,A′,F,F′,K,K′,P,P′ are shown in B,B′,G,G′,L,L′,Q,Q′, respectively. White arrowheads indicate endothelial cells. Cells were morphologically distinguished by referring to the DIC images. nt, neural tube; da, dorsal aorta. Scale bars: 10 μm

• Supplemental Figure 3 -

  Fig. S3. Elevated expression of downstream targets of the Smad2/3 pathway in Man1^Δ/Δ embryos.