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Figure 3


Fig. 3. Nodal expression is deregulated in Man1-deficient embryos. Whole-mount in situ hybridization for: (A) Nodal and (B) chordin (node), (C) Bmp4 (mesoderm), (D) cerberus-related 1 (Cer1, anterior visceral endoderm), (E) brachyury (T, posterior mesoderm), (F) Otx2 (forebrain) and Krox20 (rhombomeres 3 and 5), (G) Emx2 (telencephalon) and (H) sonic hedgehog (Shh, notochord) expression in wild-type and Man1{Delta}/{Delta} embryos. Anterior is towards the left, except for Emx2 and Nodal at E8.25 (frontal views). Arrow indicates an ectopic expression domain of Nodal; arrowheads indicate an enlarged allantois. Scale bars: 200 µm.





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