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Figure 7


Fig. 7. Abnormal fibronectin (Fn1) deposition in Man1-deficient embryos. (A) RT-PCR analyses of whole embryos at E9.5 for the expression of downstream targets of the Smad1/5 and Smad2/3 pathways. (B) Western blot of whole embryos at E9.5 for fibronectin. ß-Tubulin was used as a loading control. (C-H) Immunofluorescence analysis of the branchial arch (C,D) and trunk regions (E,F) at E9.5, and yolk sacs (G,H) at E10.0 for fibronectin. baa, branchial arch artery; nt, neural tube; VE, visceral endoderm. Arrowheads indicate endothelial cells. Scale bars: 10 µm.





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