DEV02556 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Western blot analysis of total cerebellar extracts from P0, P6 and P12 were blotted with anti-cyclin D1 or D2 antibodies. Cyclin D1 is highly expressed at P0 while cyclin D2 shows strong upregulation between P0 and P6. Anti-tubulin shows equal loading.

• Supplemental Figure 2 -

  Fig. S2. Cyclins D2 and D3 are not upregulated in the Ccnd1^−/− EGL. (A-C) At P0 cyclin D3 is not expressed in wild-type (A) or Ccnd1^−/− (B) EGL but is present in control section of developing myotubes (C). (D,E) Cyclin D2 is present in the ventricular zone of the midbrain but not the EGL of wild-type (D) or Ccnd1^−/− (E) EGL.

• Supplemental Figure 3 -

  Fig. S3. There is no significant difference in apoptosis (E, n=5) in Ccnd1^−/− cerebella (C, D) at P0 when compared with wild-type littermates (A,B). TUNEL assay of midline sections (A,C) showed only a few apoptotic cells in the posterior (B,D). Pre-neoplastic lesions (F) and medulloblastomas (H) both had apoptotic cells, while normal IGL from adjacent tissue (G,I) showed no apoptosis.

• Supplemental Figure 4 -

  Fig. S4. Migration of Ccnd1^−/− GNPs is not affected. (A-C) The wild-type EGL thickness decreases from P6 (A) to P12 (B) to P15 (C), when it is nearly absent. (D-F) The Ccnd1^−/− EGL shows a similar decrease in thickness from P6 (D) to P12 (E) to P15 (F), suggesting that GNPs are not impaired in their migration out of the EGL.