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Fig. 3. mrp-4(cd8) suppresses yolk degradation and lipid accumulation defects of cup-5(zu223). (A) Confocal micrographs of `comma' stage embryos laid by hermaphrodites carrying the YP170::GFP transgene (green in merge) and grown on plates containing LysoTracker Red (red in merge). All images of the same marker were taken with the same exposure and at the same magnification. Arrows indicate staining of intestinal cells. (B) Quantitation of the surface area of the YP170::GFP/LysoTracker Red granules in intestinal cells shown in A. One pixel is 0.01 µm<sup>2</sup>. (C) Confocal micrographs of `1.5-fold' stage embryos laid by the indicated hermaphrodites carrying the GFP::LGG-1 transgene. Long arrows indicate staining of intestinal cells. This transgene also carries a marker that expresses GFP in pharyngeal cells (short arrows). All images were taken with the same exposure and at the same magnification. (D) Confocal micrographs of wild-type, mrp-4(cd8), cup-5(zu223) or mrp-4(cd8); cup-5(zu223) `comma' stage embryos stained using the TUNEL assay to detect DNA-strand breaks. The average number of TUNEL-staining bodies in each strain is indicated at the top of each panel. (E) Confocal micrographs of `1.5 fold' stage embryos laid by hermaphrodites carrying the YP170::GFP transgene (green in merge) and stained for Nile Red (red in merge). All images of the same marker were taken with the same exposure and at the same magnification. Arrows indicate staining of intestinal cells. (F) Quantitation of the intensity per surface area of the Nile Red granules in intestinal cells shown in E. Scale bars: 10 µm in all images. The alleles in all panels are mrp-4(cd8) and cup-5(zu223).

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