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Fig. 8. The engrailed repressor domain of lbx1 is necessary for its
function. (A-F) mRNA from a mutant lbx1 construct that lacks the
engrailed homology domain was injected into one cell at the two-cell stage.
The injected sides are on the right. Phenotypes opposite of wild-type
lbx1 overexpression were observed. At stage 24, a transverse section
reveals an increase in myoD staining on the injected side (A,
arrow), while a transverse section through a stage 31 tadpole exhibits a
decrease in Pax3 staining (B, arrow). Transverse sections through stage
29 tadpoles (C-F) were stained with the pH3 antibody alone (C,E)
or with 12/101 (D,F). No increase in the number of mitotic nuclei is observed
on the injected sides (C,E), and 12/101 staining (D,F) indicates a slightly
shorter, yet wider, differentiated myotome (arrows).
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