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Fig. 9. Co-injection of myoD mRNA with lbx1 mRNA eliminates
overproliferation and pax3 upregulation. (A-O) Embryos were
injected into one cell at the two-cell stage with, lbx1
(A-C), myoD (D-F), myf5
(G-I), lbx1 + myoD (J-L) and
lbx1 + myf5 (M-O). Injected embryos were grown
until stage 29, sectioned in the transverse plane, and analyzed with
anti-phospho-histone H3 antibody (A,D,G,J,M), 12/101 (B,E,H,K,N) or
pax3 expression (C,F,I,L,O). The injected side of each tadpole is on
the right, marked by ß-galactosidase (red). Anti-phospho-histone H3 and
12/101 staining were performed on the same sections, while pax3
staining was carried out on different tadpoles. Injection of lbx1
causes an increase in proliferation (A), decrease in differentiated muscle (B)
and increase in pax3 (C). Injection of myoD causes no change
in proliferation (D), an increase in differentiated muscle (E) and a decrease
in pax3 expression (F). Injection of myf5 causes no
significant change in cell proliferation (G), a slight increase in
differentiated muscle (H) and no change in pax3 expression (I).
Injection of lbx1 + myoD (J-L) produces a phenotype
consistent with injection of myoD alone, while injection of
lbx1 + myf5 (M-O) causes a phenotype like that of
lbx1 alone.
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