DEV02198 Supplementary Material

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• Supplemental Figure 1 -

  Fig. S1. Expression pattern of Ddok protein and mRNA in wild-type (wt) embryos of different stages. (A-F) Ddok protein expression determined by immunofluorescence using Ddok antiserum. Embryos were staged according to Campos-Ortega and Hartenstein (Campos-Ortega and Hartenstein, 1997), and are oriented with anterior to left and posterior to right and laterally viewed (except F, dorsolateral, and L, dorsal). (A,B) Stage 2-3 (A) and stage 4-5 (B) are syncytial and cellular blastoderm stages, respectively. Both of these stages show heavy maternal contribution. (C,D,E) The stages of germband extension and retraction (stages 7-11), with ectodermal staining of Ddok. (F) Stages 12-14, when Ddok is expressed in the hindgut (hg) and malphigian tubules (mpg). (G-L) mRNA expression by whole mount in situ hybridization using a CO₂H-terminal Ddok antisense (digoxigenin-labeled) riboprobe. The mRNA expression pattern is similar to the protein expression pattern. Preimmune, preimmune antiserum staining of control stage 2-3 embryo. Sense, Ddok sense riboprobe control, stage 2-3 embryos. st, stomodeum; pr, proctodeum; cf, cephalic furrow; ep, epidermis; pc, pole cells; am, anterior midgut; pm, posterior midgut; tr, trachea.