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Fig. 7. Expression of tyrosine phosphorylated Shark at the periphery of
epidermal cells is decreased in Ddok mutant embryos, during DC.
(A,B) Embryo extract western blotted with (A) anti-pY927 and
anti-phosphotyrosine (anti-P-Tyr-100) antibodies, and (B) anti-pY927, before
(lane 1) and after (lane 2) phosphatase (CIAP) treatment of membrane, and with
anti-Shark antiserum (lane 3). (C,D) Staining of wild-type and
DdokPG155 mutant embryos with anti-pY927 Shark peptide
antibody. Arrowheads indicate the LE.
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