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Fig. 1. Comparison of amino acid sequences and temporal expression patterns of
Xenopus EGF-CFC factors. (A) Comparison of the amino acid
sequences of Xenopus EGF-CFC proteins (XCR1, XCR2, XCR3S and XCR3L).
XCR3L has a 72 amino acid insertion following the signal sequence of XCR3S.
Dash indicates no amino acid present. Identical residues are shadowed.
Conserved EGF-like domains and CFC domains are underlined. Cysteine residues
in the additional 72 amino acids of XCR3L are indicated by asterisks.
(B) Temporal expression patterns of Xenopus EGF-CFC genes
(XCR1, XCR2, XCR3S and XCR3L) detected by RT-PCR. RNA was
extracted from embryos at the stages indicated over each lane. XCR3S
(lower band) and XCR3L (upper band) were detected using a set of
primers designed to span the insert region. Both bands showed a similar
temporal expression pattern. e, un-fertilized egg; RT-, RT-PCR reaction
without reverse transcriptase.
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