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Fig. 6. Mapping and sequencing MNT. (A) mnt
mutations were mapped to BAC MTG10 on chromosome V. Figures below the marker
names show the number of recombination events in 1578 chromosomes scored.
(B) Scoring of an allelism test between mnt mutants and
Salk_108995 T-DNA insertion mutants by floral phenotype. (C) Scoring of
complementation by floral and seed phenotype. mnt + ARF2=T1
progeny of an mnt mutant transformed with the wild-type ARF2
gene and flanking genomic DNA. This construct restored wild-type floral (left)
and seed phenotypes (right) to the mutant. (D) The ARF2 gene
with the positions of the mnt/arf2-9 and Salk_108995/arf2-8
mutations marked. (E) Alignment of a fragment of wild-type
ARF2 cDNA with the mutated region in mnt/arf2-9 showing a
4-bp deletion. (F) Alignment of the N-terminal portion of the wild-type
ARF2 and mutant mnt/arf2-9 proteins, showing an early frameshift and stop
codon. (G) The ARF2 protein marked with the position of the stop codon
generated by the mnt/arf2-9 lesion. DBD, DNA binding domain; MR, variable
middle region; III and IV, domains involved in dimerization with other ARFs or
with Aux/IAAs (Liscum and Reed,
2002).
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