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Fig. 5. Expression of COGC-3. (A-C) Immunolocalization of COGC-3 in a
cross-section of a wild-type L4 larva. The section was stained with
anti-COGC-3 (red), fluorescein phalloidin (green) and DAPI (blue). (A)
Merged image. (B) Anti-COGC-3 image. (C) Schematic drawing of
the positions of tissues. Arrowhead indicates the perinuclear localization of
COGC-3 in a muscle cell. The muscle cells contain actin filaments stained by
fluorescein phalloidin apically and the nuclei basally. (D)
Localization of COGC-3 in a cogc-3(k181) L4 larva. No COGC-3 signal
was detected. Dorsal is towards the top. Scale bar: 12.5 µm.
(E-J) Whole-mount immunohistochemistry with COGC-3 antibody.
Antibody staining patterns (EG,I) and corresponding schematic drawings (F,H,J)
of wild-type L4 larvae. (E,F) Pharynx, (G,H) vulva and (I,J) seam cell. Dorsal
is towards the top. Anterior is leftwards. Scale bar: 25 µm.
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