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Fig. 6. Immunocytochemistry of COGC-3. (A-C) Confocal images of a body wall
muscle cell of an L4 larva transgenic for mig-23::GFP. (A)
Anti-COGC-3, (B) anti-GFP and (C) a merged image. Some of the
COGC-3 signals colocalized with MIG-23-GFP (arrowheads). (D-F) Confocal images
of a body wall muscle cell of an L4 larva transgenic for YFP::TRAM.
(D) Anti-COGC-3, (E) anti-GFP and (F) a merged image.
YFP-TRAM and COGC-3 signals colocalized at the perinuclear region (small
arrows). (G,H) Confocal images of MIG-23-GFP in body wall muscle cells in
cogc-3(k181) L4 larvae. (G) Anti-COGC-3 and (H)
anti-GFP. The number and intensity of MIG-23-GFP signals were reduced. A
similar result was obtained in cogc-1(k179) (data not shown).
(I-N) Confocal images of mRFP-SP12 (I,L) and MIG-23-GFP (J,M)
and merged images (K,N) in body wall muscle cells in wild-type (I-K) and
cogc-3(k181) (L-N) L4 larvae. MIG-23-GFP partially colocalizes with
mRFP-SP12 in wild type, but not in cogc-3 mutants. The boundaries of
the muscle cells are depicted by broken white lines. Scale bars: 25 µm.
(O) Western blot of endogenous MIG-23. Protein lysates prepared from
each strain were immunoblotted with anti-MIG-23 and anti-
-tubulin.
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