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Fig. 4. Z-stack projections from time-lapse videos of axons from Tv cells expressing EcR-B1F645A or EcR-B1W650A. (A) Z-stack time-lapse projection of two neurohemal organs (T2 above, T3 below) from cells expressing EcR-B1F645A from 20 to 23 hours apf, 60 minutes apart. No filopodia are visible during this time period. (B) Z-stack projections of remodeling axon arbors from cells expressing EcR-B1F645A. From 24 to 30 hours apf, filopodia from the partially pruned T1 (above) fasciculate and stabilize with filopodia from T2 (arrowhead). The last panel is an enlarged still of T1 at 26 hours apf, showing filopodia. (C) Z-stack time-lapse projections of axon arbors (T3 above, T2 below) from cells expressing EcR-B1W650A, from 23 to 29 hours apf, with an enlarged still at 26 hours apf, showing no filopodia.





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