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Fig. 7. The GATA sequence is necessary for intestinal gene expression. The
GATA sequence in the promoters of elo-6, gst-42 and D2030.5 was
mutated and used to generate transgenic GFP reporter strains. (+) indicates
the wild-type promoter for each gene and (GATA) indicates that one copy of the
GATA motif in the promoter was mutated. Mean pixel intensity was calculated by
averaging the image pixel intensity of 20 young adult hermaphrodite animals.
(A) Bright-field and GFP images of wild-type and mutant strains.
(B) Quantification of GFP expression indicates that the variation
between lines is less than the variation between wild-type and mutant strains
(P<0.001), indicating that the difference between wild-type and
GATA mutant expression levels is not due to variability in the transformation
procedure. There is little difference in expression between different lines
formed from the same DNA construct, not only in the five lines shown here, but
also for three other lines used in a different study (Y.L., unpublished).
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