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Fig. 3. Wnt signal-dependent regulation of enhancer N-1c. (A)
Mutations and probe sequences for Blocks A and B, used for EMSA, and
comparison with Lef1/Tcf-binding consensus. (B) EMSA performed using a
sequence B probe, with recombinant cLef1 (left) or embryo nuclear extract
(right). Arrows indicate cLef1-probe complex. (C) Effect of exogenous
expression of stabilized ß-catenin (b), Wnt antagonists Dkk1
(c) or Sfrp1 (d) on enhancer N-1c activity. Successful
electroporation of a broad embryonic area was confirmed by DsRed1 expression
(insets). Anterior margin of the embryo and the node position are indicated by
an arrow and an arrowhead, respectively. At least six electroporated embryos
were used to examine the effects of these molecules, which all gave identical
results.
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