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Fig. 6. Effects of BMP signals on activity of enhancer N-1c trimer and Sox2 expression. (Top) Embryonic Sox2 expression (in situ hybridization) and (bottom) activity of enhancer N-1/N-1c (EGFP fluorescence) are compared using the same embryos. (A) Enhancer N-1. (B) Enhancer N-1c (trimer). (C) Effect of misexpression of constitutive-active (CA) Alk6, which wiped out Sox2 expression. (D,E) Effect of misexpression of BMP antagonists cNoggin (D) or dominant-negative (DN) Alk6 (E), posteriorly extending the Sox2 expression in a manner matching the activity of enhancer N-1c.





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