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Fig. 3. Inhibiting the degradative or recycling pathways does not affect extracellular Wingless distribution. (A,B) Wg conventional staining of wing discs expressing (A) Rab7TN, (B) Rab4SN + Rab11SN (inset shows nuclei positive for activated Caspase). Compare dorsal expressing and ventral non-expressing compartments. (A',B') Wg extracellular staining of the same genotypes. The dorsal-ventral boundary is indicated by a red line. Wg extracellular distribution is not altered by any of these treatments, but note intracellular Wg accumulation in A. Scale bars 20 µm.





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