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Fig. 5. Rab5SN expression alters subcellular localization and mRNA levels of Fz2
and Arrow. (A) In-situ hybridization to Fz2 mRNA in a disc
after 4 hours 30 minutes Rab5SN expression in the posterior compartment. Inset
shows Rab5SN expression domain (green). (B) In-situ hybridizations
detecting arrow mRNA in discs of different genotypes. Left: wild
type. Middle: disc expressing GFP-Wg in the posterior compartment for 6 hours.
Right: disc expressing Rab5SN in the posterior compartment for 4 hours 30
minutes. (C) Discs expressing Rab5SN in the dorsal compartment for 5
hours 30 minutes stained for Arrow, Fz2 and Armadillo to mark apical
junctions. Apical, middle sections and the basal region are shown. Note the
accumulation of Fz2 and Arrow above the junctions. Large arrows in middle
sections indicate the apical cell surface. In the basal region, the wing pouch
curves so that the apical-basal axes of the epithelial cells at the edges of
the wing pouch lie parallel to the focal plane. Thus, a complete longitudinal
section of these cells is visible. Small arrows point to their basal sides.
(D) Larger magnification of the areas boxed in C. Armadillo staining
reveals the apical junctions of two rows of epithelial cells facing each
other. Fz2 and Arrow accumulate above cellular junctions in Rab5SN-expressing
tissue. (E) Single confocal section of a wing imaginal disc expressing
YFP-Rab7, CFP-Rab5 and stained for Arrow. Arrowheads indicate co-localization
between Arrow and Rab5/7 endosomes. Quantifying ten confocal sections
corresponding to the apical-most 10 µm of the disc shown indicates that 49%
of the brightest Arrow spots co-localize with either YFP-Rab7 or CFP-Rab5.
Scale bar: 20 µm.
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