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Fig. 6. Rab5SN expression alters subcellular localization and mRNA levels of Dlp. (A,B) Wing disc after 5 hours 30 minutes Rab5SN expression in dorsal compartment, stained for Dlp. Dlp accumulates more strongly apically (A) than basal-laterally (B) in response to Rab5SN expression. (C) In-situ hybridization to dlp mRNA in a wild-type disc (top) and a disc after 4 hours 30 minutes Rab5SN expression in the posterior compartment (to the right). (D-G) Single confocal section of a disc stained for endogenous Dlp and expressing the indicated FP-Rab proteins. Quantifying ten confocal sections corresponding to the apical-most 10 µm of this disc indicates that 19% of the brightest Dlp spots co-localize with either YFP-Rab7 or CFP-Rab5. D, dorsal compartment; V, ventral compartment.





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