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Fig. 2. Experimental protocol. (A) Immature oocyte. (B) The immature oocyte is bisected manually with a fine glass needle into a GV-containing and a non-nucleate fragment. (C) The GV-containing fragment is enucleated with a micropipette. This fragment is used as a centrosome donor. (D) Actinase-treated immature oocyte that is treated with 1-methyladenine (1-MeAde) to induce maturation. (E) Mature egg bearing both PB1 and PB2. (F) Conjugate of a non-nucleate fragment and a mature egg. Scale bar: 50 µm.





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