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Fig. 8. Molecular analysis of SHH signaling in Raldh2-/- embryos. Whole-mount in situ hybridization with Shh (A-D), Ptch1 (E,F), Gli1 (G,H), Gli3 (I,J) and Olig2 (K,L) probes. Embryos are at E7.75 (A,B), E9.5 (C,D) and 12-15 somites (E-L). Profile views, except in A,B (ventral views). Insets show transverse sections of the infundibulum (red brackets, C,D), ventral views of the same embryos (G,H) or details of the Olig2-positive region in older (E9.5) embryos (K,L). Genotypes are indicated above. ov, optic vesicle; pp, prechordal plate; te, telencephalon.





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