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Fig. 4. Transcriptional regulation by p53 in neural stem cells. (A)
Volcano plot showing the relative expression levels (%) of genes in
p53-/- compared with wild-type neurospheres in relation to
the statistical significance of the difference. The cDNA arrays contain two
independent p21 probes, which is why this gene appears as two independent
measurements. (B) Categorization of dysregulated genes by gene ontology
themes indicates overrepresentation of genes regulating proliferation. Green
indicates upregulation and red indicates downregulation. (C)
Quantitative RT-PCR analysis of the relative (%) mRNA expression of Cip/Kip
family members. (D) Representative examples of western blot analysis of
p21 protein levels in dissected LVW from wild-type and
p53-/- mice. (E) Scheme for genes controlling stem
cell self-renewal converging on p53. Green circles indicate genes implicated
as positive regulators of self-renewal; red circles indicate putative negative
regulators.
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