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Figure 2


Fig. 2. PIN1 acts redundantly with AS1 to exclude BP expression from leaves and promote lateral organ initiation. (A-D) Rosettes just before bolting of (A) Col, (B) pin1-6, (C) as1-1 and (D) pin1-6;as1-1. c, cotyledon. (E,F) The number of rosette leaves in (E) Col (n=68), as1-1 (n=56), pin1-En134 (n=30) and pin1-En134;as1-1 (n=19; note, pin1;as1 double mutants were never observed to flower earlier than pin1 or as1 single mutants), and (F) Col (n=68), bp-9 (n=17), pin1-En134 (n=30) and pin1-En134;bp-9 (n=5) plants. (G-L) Inflorescences of (G) Col, (H) bp-9, (I) blr-126, (J) pin1-6, (K) pin1-6;bp-9 and (L) pin1-6;blr-126. (M) The number of naked branches lacking flowers as a fraction of the total number of branches counted for pid (n=28) and bp;pid (n=29) mutants. (N) Quantitative RT-PCR analysis of BP and AS1 expression in mature leaves of Col (white bars) and pin1-En134 (black bars) plants. Error bars indicate s.e.m. (O-R) GUS-stained seedlings of BP::GUS grown on (O) MS media and (P) MS media supplemented with 20 µM TIBA (arrowheads indicate ectopic expression of BP::GUS in leaves), and STM::GUS grown on (Q) MS medium and (R) MS medium supplemented with 20 µM TIBA. Scale bars: 1 cm in A-D,G-L; 200 µm in O-R.





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