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Fig. 3. PIN1 regulates leaf margin development. (A,B)
Half-leaf silhouettes of the seventh rosette leaf of (A) wild type and (B)
pin1-En134. (C,D) Confocal micrographs of GFP
expression (green) in wild-type developing leaf margins. (C)
DR5rev::GFP expression maximum at the tip of a serration, and (D)
PIN1:GFP localisation indicates the direction of auxin flux (arrowheads)
towards the serration tip. (E,F) Whole-leaf silhouettes of the
seventh rosette leaf of wild-type (Col) plants grown on (E) MS medium and (F)
MS medium supplemented with 5 µM NPA. (G-I) Confocal micrographs of
GFP expression (green) in developing leaf margins indicated by boxes in E,F.
(G) PIN1:GFP localisation indicates the direction of auxin flux (arrows)
towards the serration tip in plants gown on MS (box in E), whereas, in the
margin of NPA-treated plants (box in F), PIN1:GFP localisation is non-polar
(H) and DR5rev::GFP expression is diffuse (I). Red indicates
chlorophyll autofluorescence. Scale bars: 0.5 cm in A,B,E,F; 20 µm in C,D;
10 µm in G-I.
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