DEV02583 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Cell death is increased specifically in the dorsal neural epithelium following Sema3d knockdown. (A-H) Acridine Orange labeling of cell death at 24 hpf in the dorsal hindbrain (A,B), ventral hindbrain (C,D), eye (E,F) and spinal cord (G,H) in CONMO (A,C,E,G) and 3DMO (at 250 μM) (B,D,F,H) injected embryos. (A-F) Dorsal views, anterior is leftwards. (G,H) Lateral view, anterior is leftwards. Scale bar: 40 μm for A-H.

• Supplemental Figure 2 -

  Fig. S2. Rescue of the Sema3D splice morpholino (3DI4E5MO) by Sema3D^myc overexpression. (A-F) In situ hybridization for cyclin D1 in the hindbrain at 17 hpf in Sema3D^myc transgenic embryos (A-D) or wild-type embryos (E,F) that were uninjected (A,B) or injected with 3DI4E5MO (C-F) and were either not heat-shocked (A,C,E) or heat-shocked for 1 hour at 14 hpf (B,D,F). (G,H) Quantification of the number of BrdU (I) and PH3 (J) labeled nuclei in the neuroepithelium of rhombomeres 3-5. Numbers in parentheses indicate n. *P<0.005 versus uninjected NHS, ^#P<0.03 versus wild-type HS. oto, otocyst. (A-F) Dorsal views, anterior is leftwards. Scale bars: 40 μm for A-F.