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Fig. 1. Sema3d mRNA is expressed in NCCs and morpholino knockdown of Sema3d
disrupts NCC derivatives without affecting NCC induction. (A-C) In
situ hybridization for sema3d at 15 hpf (A,B) and 22 hpf (C).
(D-F) Alcian Blue labeling of pharyngeal cartilage at 5 dpf;
(G-I) DIC imaging of melanophores in 2 dpf in CONMO (D,G), 3DMO (E,H)
and 3DI4E5MO (F,I) injected embryos. Arrow in G indicates ventral horn of
melanophores. (J) RT-PCR on mRNA extracted from embryos injected with
Sema3d splice blocking morpholinos. (K-N) In situ hybridization for
crestin at 13 hpf (K,L) and hoxb2a at 18 hpf (M,N) in CONMO
(K,M) and 3DMO (L,N) injected embryos. (A,K-N) Dorsal views, anterior is
leftwards. (B) Transverse section through rhombomere 4. (C,G-I) Lateral views,
anterior is leftwards. (D-F) Ventral views, anterior is leftwards. r2-r5,
rhombomeres 2-5; oto, otocyst; m, hs, ch and gill, Meckel's, hyosymplectic,
ceratohyal and gill cartilages, respectively; bp, base pairs. Scale bars: 40
µm for A,C,M,N; 20 µm for B; 100 µm for D-I; 80 µm for K,L.
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