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Figure 5


Fig. 5. {Delta}TCFgfp disrupts cell cycle and crestin expression and eliminates cyclin D1 and sema3d expression. (A-I) Immunodetection of BrdU (A,B,I) and in situ hybridization for crestin (C,D), cyclinD1 (E,F), sema3d (G,H) in the hindbrain at 17 hpf of heat-shocked wild-type siblings (A,C,E,G), {Delta}TCFgfp transgenic (B,D,F,H), and {Delta}TCFgfp + Sema3dmyc double-transgenic (I) embryos. (J) Quantification of the number of BrdU-labeled nuclei in the neuroepithelium of rhombomeres 3-5. Numbers in parentheses indicate n. *P<10-13 versus wild-type sibling, #P<10-4 versus {Delta}TCFgfp, t-test. (A-I) Dorsal views, anterior is leftwards. oto, otocyst. Scale bar: 40 µm for A-I.





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