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Fig. 2. Defects in prethalamus and thalamus development and rostral expansion of
the pretectum in Fez-/-Fezl-/- embryos at
E12.5. A-T) Expression of Dlx1 (marker for prethalamus and
ganglionic eminence, A-D), Gbx2 (thalamus, E-H), Lhx1
(posterior pretectum and ZLI, I-L), Ebf1 (anterior pretectum, M-P)
and Sox14 (rostral domain of thalamus, Q-T) in control,
Fez-/-Fezl+/-,
Fez+/-Fezl-/- and
Fez-/-Fezl-/- embryos was analyzed by in situ
hybridization. Sagittal sections with anterior to the left. Dlx1
expression was not detected in the prethalamus but was maintained in the
hypothalamus and ganglionic eminence in
Fez-/-Fezl-/- embryos (D). Gbx2
expression was strongly reduced and detected in patches (arrows in H) in
Fez-/-Fezl-/- embryos. Lhx1 expression
was absent in the ZLI, but was not significantly affected in the posterior
pretectum of Fez-/-Fezl-/- embryos (I-L). The
expression domain of Ebf1 was expanded rostrally in
Fez-/-Fezl-/- embryos (P), compared with that
in the wild-type, Fez-/-Fezl+/- and
Fez+/-Fezl-/- littermates (M-O). The expression
of Sox14 was abolished in
Fez-/-Fezl-/- embryos (T). apt, anterior
pretectum; GE, ganglionic eminence; ht, hypothalamus; ppt, posterior
pretectum; pth, prethalamus; th, thalamus.
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