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Fig. 3. Defects in telencephalon formation and regionalization of the
diencephalon in Fez-/-Fezl-/- embryos.
Expression of Emx2 (A-D), Pax6 (E-H),
Dlx1 (I-L), Shh (M-P), Tcf4 (Q-T)
and Lhx5 (U-X) in control (A,E,I,M,Q,U),
Fez-/-Fezl+/- (B,F,J,N,R,V),
Fez+/-Fezl-/- (C,G,K,O,S,W) and
Fez-/-Fezl-/- embryos (D,H,L,P,T,X) at E10.5.
(A-T) Whole-mount in situ hybridization and lateral views of anterior
neuroectoderm, with anterior to the left. (U-X) Coronary sections of the
diencephalon. Emx2 expression in the dorsal telencephalon was reduced
in Fez-/-Fezl-/- embryos. Pax6
expression was downregulated in the thalamus at this stage (marked by dots,
E,F,G) in control, Fez-/-Fezl+/- and
Fez+/-Fezl-/- embryos, but not in
Fez-/-Fezl-/- embryos (arrow, H). The
expression domain of Pax6 in the dorsal telencephalon was reduced but
not significantly affected in the diencephalon except for the thalamus (region
between arrowheads) in Fez-/-Fezl-/- embryos.
Dlx1 expression was detected in the ganglionic eminence (arrow, I,L)
but not in the prethalamus (arrowhead, I,L) in
Fez-/-Fezl-/- embryos. Shh expression
was detected in the AEP, but not in the ZLI in
Fez-/-Fezl-/- embryos (arrow, P), compared with
controls (M-O). The domain expressing Tcf4 at a high level (thalamus
and pretectum region, marked by dots) was expanded in
Fez-/-Fezl-/- embryos (T), compared with
controls (Q-S). Lhx5 expression was abolished in the prethalamus of
Fez-/-Fezl-/- embryos (X). ht, hypothalamus;
pth, prethalamus; th, thalamus.
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