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Fig. 4. Reduction of rostral diencephalon and expansion of caudal diencephalon
in Fez-/-Fezl-/- embryos at E9.5.
Expression of Emx2 (A,B), Pax6
(C,D), Fgf8 (E,F), Wnt3a
(G,H) and En2 (I,J) in control
(A,C,E,G,I) and Fez-/-Fezl-/- embryos
(B,D,F,H,J) was analyzed by whole-mount in situ hybridization. Lateral views
of anterior neuroectoderm, with anterior to the left. Expression of
Emx2 (A) and Pax6 in the dorsal telencephalon (between
arrowheads, C) was reduced, but the Pax6 expression in the
diencephalon (marked by dots) was maintained in
Fez-/-Fezl-/- embryos. Fgf8 expression
in the commissural plate and mid-hindbrain boundary was maintained, but that
in the dorsal prethalamus was absent (arrowheads E,F) in
Fez-/-Fezl-/- embryos. The expression domain of
Wnt3a was caudal to the ZLI in control embryos (G) and was expanded
rostrally in Fez-/-Fezl-/- embryos (arrow in
H). Expression of En2, which marks the midbrain, was not affected in
Fez-/-Fezl-/- embryos (I,J). cp, commissural
plate; mhb, mid-hindbrain boundary; pth, prethalamus.
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