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Figure 7


Fig. 7. Enhancer and promoter region of Fezl. (A) Schematic diagram of the Fezl enhancer/promoter and the constructs used for transgenesis. (B-D) Detection of lacZ expression by X-gal staining in Fezl8.2p-lacZ (B) and Fezl2.7p-lacZ (C,D) transgenic mouse embryos, in which lacZ expression was driven by the 8.2 kbp and 2.7 kbp Fezl enhancer/promoter, respectively. (B,C) E8.5 embryos, lateral views with anterior to the top. (D) E9.5 embryo, lateral views of the anterior neuroectoderm. The 8.2 kbp Fezl enhancer/promoter recapitulated the endogenous Fezl expression (rostral to the ZLI), whereas the 2.7 kbp Fezl enhancer/promoter showed a caudally expanded expression of lacZ (arrows, D).





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