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Fig. 2. Polo localization during the early steps of oogenesis. (A)
Schematic representation of a Drosophila germarium focusing on oocyte
determination and meiosis progression. (B) General view of GFP-Polo
expression (green) in a germarium and a stage 2 follicle. Arrowheads indicate
Polo spots in region 2 and 3. (C-E) Enlargements of (B). (C) In region
1, Polo is found in many dots in each interphase germline cell, but is not
colocalized with centrosomes (CP309, red). (D) In region 2a, Polo dots are
progressively restricted to the pro-oocytes but do not show colocalization
with BicD (blue) or centrosomes (red). (E) In region 3, Polo is found at the
anterior or lateral part of the oocyte whereas BicD and centrosomes are in the
posterior region. (F,G) A germarium expressing GFP-Polo (green)
stained for SCs [C(3)G, red] and fusome and follicular cells (Hts, blue). Polo
concentrates in meiotic cells from region 2a (F) to region 3 / stage 1
follicle (G). (H) Single confocal section of cysts in region 2 showing
that GFP-Polo (green) is not localized on the remnant fusome (red).
(I,J) Endogenous Polo protein (green) co-stained with SCs (red)
shows a similar dynamic localization to GFP-Polo in regions 1 (I), 2 and 3 (J)
of the germarium. (K) polo mRNA in situ hybridization. Strong
expression of polo is detected in the germline in region 1 of
germarium (arrow) and in follicle cells in region 2a (arrowhead).
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