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Fig. 5. phox2a, gata2 and tfap2a expression in
cervical sympathetic ganglia of wild type and hands off mutants.
Whole-mount in situ hybridizations viewed from dorsal.
(A,D,G,J) phox2a-labeled sympathetic
ganglia (dashed circle) and enteric neurons (white arrowheads). Control and
hands off embryos show the same expression of phox2a at 2
dpf (A,D) and 3 dpf (G,J). gata2 expression is strongly reduced in
hands off embryos (E,K) compared with wild-type embryos
(B,H). tfap2a expression is massively reduced in
hands off embryos (F,L) compared with wild type
(C,I). (M) Area of phox2a-expressing cells at 2
and 3 dpf in hands off mutants compared with wild type. (Number of
embryos analysed: seven wild type, three mutant for 2 dpf; ten wild type, five
mutant for 3 dpf.) (N) Area of gata2-expressing cells at 2 and
3 dpf in hands off mutants compared with wild type. (Number of
embryos analysed: six wild type, five mutant for 2 dpf; 12 wild type, nine
mutant for 3 dpf.) (O) Area of tfap2a-expressing cells at 2
and 3 dpf in hands off mutants compared with wild type. (Number of
embryos analysed: five wild type, 14 mutant for 2 dpf; 15 wild type, four
mutant for 3 dpf.) Data are presented as mean ± s.e.m.; n.s. not
significantly different (**P<0.01,
***P<0.001). Phox2a staining of epibranchial
placodes is indicated by asterisks.
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