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Fig. 1. Neuromuscular terminals are missing and mislocalized in piranha
mutant third instar larvae. Arrows illustrate the normal localization of
NMJs on muscles of wild-type larvae. (A) Schematic diagram of the
innervation pattern of the somatic musculature of an abdominal hemisegment
viewed from the exterior. The approximate branching pattern of the five motor
nerves and the innervation sites are superimposed onto the muscle pattern. The
approximate locations of motoneurons in the ventral nerve cord are indicated.
(B-D) Innervation defects on dorsalmost muscles. (B) Schematic diagram
and (C) confocal micrograph of NMJs (bright green) on muscle pairs 1/9 and
2/10 in undissected wild-type larvae as seen with the CD8-GFP-Sh marker
(exterior view). (D) NMJs are missing on muscle 1 and reduced to a remnant on
muscle 9 in this
piranhaD427/piranhaK788 mutant
hemisegment. (E-G) Innervation defects on ventral muscles. (E)
Schematic diagram and (F) confocal micrograph depicting the locations of NMJs
on ventral internal muscles 12, 13, 6 and 7 in an undissected wild-type larva
expressing CD8-GFP-Sh. (G) In this
piranhaD427/piranhaK788 mutant
hemisegment, NMJs are missing on muscle 13 and rudimentary in the cleft of
muscles 6/7. Muscle 12 displays innervation defects as well. Asterisks
indicate NMJs and muscles outside of the intended focal plane. (H-M)
Confocal images of the ventral innervation pattern of a dissected wild-type
(H-J) and piranhaD427/piranhaK788
mutant larva (K-M) viewed from the interior. NMJs on muscles 12, 13, 6 and 7
are visualized with CD8-GFP-Sh (H,K) and anti-Dlg (I,L). Endogenous Dlg
staining completely overlaps with transgenic CD8-GFP-Sh (J,M). Note the
altered innervation pattern of muscles 12 and 13 and the lack of NMJs in the
cleft between muscles 6 and 7 in the mutant animal (arrows in K-M). Dorsal is
up and anterior is left in all figures. AC, anterior commissure; PC, posterior
commissure.
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