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Fig. 1. Identification of tud missense mutations. (A)
Missense and nonsense mutations are placed at their corresponding locations
within Tud protein sequence. Tud domains are indicated by blue squares.
(B) Western blot of ovary extracts from wild type (wt) and tud
mutants probed with TUD-A63 antibody and anti-Dynein antibody to detect Dynein
as a loading control. (C) Tud proteins and Dynein were quantified by
western blot with TUD-A63 antibody and anti-Dynein antibody, respectively (see
Materials and methods). Tud signals were normalized to the Dynein signals and
the average percentage relative to the wild-type ±s.e.m. is recorded.
For each table entry, 3-4 measurements (n) were used to calculate the
average percentage.