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Figure 1


Fig. 1. Identification of tud missense mutations. (A) Missense and nonsense mutations are placed at their corresponding locations within Tud protein sequence. Tud domains are indicated by blue squares. (B) Western blot of ovary extracts from wild type (wt) and tud mutants probed with TUD-A63 antibody and anti-Dynein antibody to detect Dynein as a loading control. (C) Tud proteins and Dynein were quantified by western blot with TUD-A63 antibody and anti-Dynein antibody, respectively (see Materials and methods). Tud signals were normalized to the Dynein signals and the average percentage relative to the wild-type ±s.e.m. is recorded. For each table entry, 3-4 measurements (n) were used to calculate the average percentage.





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