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Figure 2


Fig. 2. Specificity of mtx1 MOs. (A) Schematic diagram of mtx1 genomic structure and position of mtx1 MOs. mtx1 has three exons (blue boxes) and two introns. Two independent ATG MOs, MO(A) and MO(B), and a splice donor MO, MO(C), caused essentially the same phenotype when injected into the YSL at the 1000-cell stage. The splice donor MO, mtx1 MO(C), leads to a 20 bp insertion in mtx1 mRNA, which leads to a frame shift and premature termination. Green arrowheads indicate the position of the primers used for the RT-PCR analysis shown in B. (B) RT-PCR analysis shows that an additional 20 bp are integrated into mtx1 mRNA in mtx1 MO(C)-injected embryos.





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