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Fig. 7. Regional specification and ECM formation in mtx1 MO-injected
heterozygous natter embryos. (A,B) Dorsal views of
hindbrain, (C,D) somite and (E,F) heart regions.
pax-b and krox20 expression in the midbrain-hindbrain
boundary and rhombomeres 3 and 5 at 19.5 hpf (A,B), and myod
expression in the segmented somites and adaxial cells at 19.5 hpf (C,D),
appeared unaffected in mtx1 MO-injected heterozygous natter
embryos. (E,F) cmlc2 expression in the myocardial cells at 22 hpf
reveals that mtx1 MO-injected heterozygous natter embryos
displayed cardia bifida. (G-L) Laminin immunostaining at 24 hpf.
Laminin deposition appeared to be greatly reduced in the hindbrain region of
mtx1 MO-injected natter heterozygous embryos (H), but not in
the trunk and tail (J). (K,L) Transverse sections of embryos (anterior region)
immunostained for ß-catenin (red) and Laminin (green). Laminin deposition
was greatly downregulated and the head structure collapsed in mtx1
MO-injected heterozygous natter embryos (L). (M-Q) Lateral
views of bright field images at 30 hpf, anterior to the left. Embryos injected
with laminin c1 MO at the 1-cell stage exhibited shortened body axis
and defects in notochord differentiation (M). Embryos injected with
lamimin c1 MO at the 1-cell stage and mtx1 MO(A) into the
YSL at the 1000-cell stage showed enhanced phenotypes in the hindbrain region
(N) or entire head region (O). Homozygous natter mutant embryos
injected with laminin c1 MO (P,Q).
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