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Fig. 2. Early mesodermal expression of tin is sufficient to specify the
dorsal mesoderm. Wild-type (left) and tin-null mutant embryos
[homozygous for Df(3L)GC14] carrying the transgene tin-AB
(right); (A-D) Lateral views; (E-H) Dorsal views. (A,B) Bin
protein in visceral muscle progenitors of stage 10 control and tin
mutant embryos carrying tin-AB. (C,D) Detection of
bkh mRNA (green), Eve (red) and Tin (blue) protein. In late stage 11
control embryos (C), bkh-expressing cardioblasts (white arrowheads)
and pericardial progenitors (red arrowhead, bkh+Eve+)
are detected within the Tin domain. In corresponding tin-AB, tin
mutant embryos (D), tin-AB-derived Tin has vanished but most
bkh- and Eve-expressing cardiac progenitors are formed.
(E,F) Staining for Odd, Zfh1 and Eve proteins identifies
Odd-(Odd-pc, yellow) and Eve-pericardial cells (Eve-pc, pink) in stage 16
embryos. In the mutant (F), both types of pericardial cells are present,
albeit arranged irregularly. The lymph gland (lg) is strongly reduced.
(G) Mef2 staining of wild-type embryo at stage 16 (dv, dorsal vessel;
sm, somatic musculature). (H) Mef2-expressing cardioblasts are present
in tin-AB, tin-, although at reduced numbers (arrowheads
indicate interruptions in the dv, which can occur at variable AP
positions).
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