QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 3. Formation and differentiation of the dorsal vessel in the absence of cardiac Tin. Dorsal views of stage 15-16 embryos, with the left column showing wild-type control embryos and the right column homozygous tin-null mutant (tin³⁴⁶) embryos carrying tin-ABD. (A) Control embryo stained for mid RNA and for Mef2 protein. (B) tin-ABD, tin^- embryo with normal mid and Mef2 expression in cardioblasts. (C) Wild-type expression of Hand mRNA in Mef2⁺ cardioblasts (cb), Mef2^- pericardial cells (pc), and the lymph gland (lg). (D) tin-ABD, tin^- embryo expressing Hand mRNA in the developing dorsal vessel. Hand in cardioblasts is present, albeit at reduced levels when compared with pericardial cells and wild-type cardioblasts. (E) Control embryo doubly stained for Mef2 and Tropomyosin (posterior portion of the dorsal vessel). (F) Tropomyosin is present in myocardial cells of tin-ABD, tin^- embryo. (G) Staining for the transmembrane protein Toll and Mef2 shows a characteristic alternation between more cubical `working' myocardial cells and elongated pairs of ostial cells (brackets) in the `heart' region of the dorsal vessel. (H) tin-ABD, tin³⁴⁶ mutant embryo stained as in G showing disorganized tube formation and lack of the correct alternation of cell shapes in the `heart' (bracket). Mef2 channels in G,H to identify cardioblasts were omitted in the higher magnification insets.

Return to article