DEV02566 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Horizontal cells are located away from the Müller cell column. (A-F) Confocal images of flat-mounted E16 retina expressing the exogenous mEGFP. The optical sections were obtained at the level of horizontal cell layer (A,B), outer plexiform layer (C,D) and outer limiting membrane (E,F). A,C,E and B, D, F are taken from the same field. A substantial proportion of the labeled horizontal cells are positioned away from the Müller cells, which enables an observation of a detailed morphology at the single cell level. Arrows and arrowheads indicate isolated horizontal cells and Müller cell columns, respectively. (E) Pie chart of the number of isolated horizontal cells and ones within the Müller cell column from 12 independent retinal regions.

• Supplemental Figure 2 -

  Fig. S2. cN390Δ CBR (−) does not change dendritic field size of horizontal cells. (A-H) Morphology of horizontal cells expressing full-length N-cadherin (A-D; the same figure as in Fig. 6A-D) or cN390Δ CBR (−) (E-H) visualized by mEGFP expression. Projection images and optical vertical sections are shown in upper and lower panels, respectively. All figures are shown at the same scale. Scale bar: 10 μm.

• Supplemental Figure 3 -

  Fig. S3. cN390Δ CBR (−) does not affect synaptic organization of horizontal cell terminals. (A-C) Morphology of type I (A), type II (B) and type III (C) horizontal cells expressing cN390Δ CBR(−). Projection images and optical vertical sections are shown in upper and lower panels, respectively. The dendrite terminals of type I/III and type II horizontal cells correctly target to the PNAL-positive (A,C) and PNAL-negative (B) sublamina, respectively. (D-G) Higher magnification views of single optical sections at the level of double cone pedicles. The dendrite terminals of horizontal cells expressing cN390Δ CBR(−) are shown in green, and the synaptic markers PNAL (D,F) and GluR4 (E,G) are shown in magenta. The dendrite terminals of type I horizontal cells normally project to both principal (arrows in D) and accessory cone pedicles (arrowheads in D). The dendrite terminals of type III horizontal cells also correctly projected to principal cone pedicles (arrows in F). GluR4 accumulates at the dendrite terminals of type I (arrows and arrowheads in E) and type III (arrows in F) horizontal cells. Scale bars: 10 μm in A-C; 5 μm in D-F.