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Fig. 1. A transposon-mediated gene transfer system that enables conditional gene
expression in the developing chicken retina. (A) Schematic drawing
of conditional gene expression. The gene cassettes flanked by the
Tol2 cis-sequence (Tol2) are integrated into the host genome by the
transient expression of Tol2 transposase (T2TP). Only the cells that
stably incorporated both CAG-rtTA-M2 and TRE-mEGFP cassettes express mEGFP
upon the addition of doxycycline (DOX), which enhances the binding of rtTA-M2
to its cis-element TRE. (B,C) Exogenous mEGFP expression (green)
in horizontal cells expressing Prox1 (B) and Pax6 (C) (magenta). Scale bars:
20 µm.
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