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Fig. 5. XHes2 promotes extra Müller cell production by affecting both glial
cell birthdate and retinal precursor proliferation. (A-M) Analysis of BrdU
incorporation (3 hour pulse) in retinal clones, following GFP or GFP
plus XHes2 lipofection. (A) Percentage of BrdU-positive cells
among GFP-transfected retinal cells (76, 509, 707 and 544 cells for the
control; 124, 347, 1058 and 322 for XHes2 at stages 30, 33/34, 35/36 and
37/38, respectively). (B-M) Typical sections of stage 33/34 (B-G) or
37/38 (H-M) retinas immunostained for both GFP and BrdU, following lipofection
of GFP alone (B-D,H-J) or GFP plus XHes2 (E-G,K-M).
At stage 37/38, BrdU-positive cells in the control are mostly restricted to
the CMZ, while many XHes2-overexpressing cells are still proliferating in the
central retina (compare arrows in I and L). (N-Q) Birthdating experiments.
Embryos lipofected with either GFP alone or GFP plus
XHes2 were injected with BrdU every 8 hours from stage 34 to stage
41. (N) Percentage of BrdU-positive cells among GFP-transfected
Müller cells (22 Müller cells for the control and 41 for XHes2).
(O-Q) An example of GFP and BrdU double staining following
XHes2 overexpression. Arrows indicate GFP-positive Müller cells
that are BrdU negative. Abbreviations: GCL, ganglion cell layer; INL, inner
nuclear layer; LE, lens; ONL, outer nuclear layer. Values are given as
mean±s.e.m. *P<0.05,
***P<0.001 (Student's t-test).
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