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Figure 7


Fig. 7. XHes2 may inhibit neurogenesis by several repression modes. (A-F) Whole-mount in situ hybridization performed on stage 14/15 embryos co-injected with either XNgnr1 or NeuroD and XHes2. XHes2 counteracts the N-tubulin inducing activity of both XNgnr1 (B, 100%, n=19; A, 0%, n=17) and NeuroD (F, 95%, n=19; E, 0%, n=20), as well as the NeuroD-inducing activity of XNgnr1 (D, 100%, n=18; C, 7%, n=15). Embryo orientation: dorsal views, anterior downwards. Abbreviations: is, injected side; nis, non-injected side. (G,H) In vivo co-immunoprecipitation experiments performed on animal cap extracts, following misexpression of tagged XHes2 and bHLH proteins. H and L indicate the position of antibodies heavy and light chains, respectively.





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